Hydrophobic Motif Site - phosphorylated Protein Kinase C β II between mTORC 2 and Akt 1 Regulates High Glucose - induced Mesangial Cell Hypertrophy
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چکیده
1 Regulates High Glucose-induced Mesangial Cell Hypertrophy 2 3 Falguni Das, Nandini Ghosh-Choudhury, Meenalakshmi M. Mariappan, Balakuntalam S. 4 Kasinath and Goutam Ghosh Choudhury 5 6 VA Research and Geriatric Research, Education and Clinical Research, South Texas Veterans 7 Health Care System, San Antonio, Texas and Departments of Medicine and Pathology, 8 University of Texas Health Science Center at San Antonio, Texas. 9 10 Running Head: PKCβII regulates mTORC1 11 12 #Address correspondence to: 13 Goutam Ghosh Choudhury, Department of Medicine, University of Texas Health Science Center 14 at San Antonio, Mail Code 7882. 7703 Floyd Curl Drive, San Antonio, Texas 78229-3900. 15 E.mail: [email protected] 16 17 18 Articles in PresS. Am J Physiol Cell Physiol (January 6, 2016). doi:10.1152/ajpcell.00266.2015
منابع مشابه
Hydrophobic motif site-phosphorylated protein kinase CβII between mTORC2 and Akt regulates high glucose-induced mesangial cell hypertrophy.
PKCβII controls the pathologic features of diabetic nephropathy, including glomerular mesangial cell hypertrophy. PKCβII contains the COOH-terminal hydrophobic motif site Ser-660. Whether this hydrophobic motif phosphorylation contributes to high glucose-induced mesangial cell hypertrophy has not been determined. Here we show that, in mesangial cells, high glucose increased phosphorylation of P...
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The pro-survival kinase Akt requires phosphorylation at two conserved residues, the activation loop site (Thr-308) and the hydrophobic motif site (Ser-473), for maximal activation. Previous reports indicate that mTORC2 is necessary for phosphorylation of the hydrophobic motif and that this site is not phosphorylated in cells lacking components of the mTORC2 complex, such as Sin1. Here we show t...
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متن کاملAkt/protein kinase B is regulated by autophosphorylation at the hypothetical PDK-2 site.
The function of Akt (protein kinase B) is regulated by phosphorylation on two sites conserved within the AGC kinase family: the activation loop (Thr-308) in the kinase core and a hydrophobic phosphorylation site on the carboxyl terminus (Ser-473). Thr-308 is phosphorylated by the phosphoinositide-dependent kinase-1, (PDK-1), whereas the mechanism of phosphorylation of the hydrophobic site, tent...
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